Bone Marrow Suppression by c-Kit Blockade Enhances Tumor Growth of Colorectal Metastases through the Action of Stromal Cell-Derived Factor-1
Figure 4
Hematoxylin-eosin staining of CT26.WT-GFP tumors shows solid tumor growth 14 days after tumor cell implantation within the dorsal skinfold chamber. Sections display tumor infiltration through the underlying muscular layer (marked by arrows) after pretreatment with anti-c-Kit (a) and lack of muscular infiltration after pretreatment with anti-c-Kit and additional neutralization of SDF-1 (b). Quantitative analysis of tumor cell invasion of the muscular layer is given as percentage of the length of the tumor. Tumors of animals pretreated with anti-c-Kit showed a significantly pronounced infiltration of the muscular layer compared to controls. Of interest, blockade of SDF-1 after anti-c-Kit pretreatment abrogated this anti-c-Kit-associated increase of tumor cell infiltration. Mean ± SEM; * versus control; # versus anti-c-Kit. Original magnification (a, b) ×88.
