Bone Marrow Suppression by c-Kit Blockade Enhances Tumor Growth of Colorectal Metastases through the Action of Stromal Cell-Derived Factor-1
Figure 5
PCNA immunohistochemistry in CT26.WT-GFP tumors at day 14 after tumor cell implantation. Animals were pretreated with anti-c-Kit (cKit-Ab, (a)) alone or anti-c-Kit and anti-SDF-1 neutralizing antibodies (cKit/SDF1-Ab, (b)). Animals treated with an isotype-matched control antibody served as controls (control). Quantitative analysis demonstrated that anti-c-Kit pretreatment significantly increased the rate of proliferating tumor cells compared to controls (c). Of interest, additional neutralization of SDF-1 significantly decreased the amount of proliferating tumor cells compared to controls and anti-c-Kit-treated animals. Mean ± SEM; * versus control; # versus anti-c-Kit. Original magnification (a, b) ×175.
