Bone Marrow Suppression by c-Kit Blockade Enhances Tumor Growth of Colorectal Metastases through the Action of Stromal Cell-Derived Factor-1

Table 2

Tumor cell migration in dorsal skinfold chambers of control animals, animals pretreated with anti-c-Kit, and animals pretreated with anti-c-Kit followed by anti-SDF-1 treatment during a 14-day observation period. Migrated tumor cells next to the tumor margin were observed from day 5 until the end of the observation time. Comparing all three groups, no significant differences could be observed concerning the total number of migrated cells as well as their distance [μm] from the tumor margin. Of interest, at the later time points, tumor cells were found at greater distances from the tumor margin.

	Time	Control	Anti-c-Kit	Anti-c-Kit/anti-SDF-1
Number	d5	22.08±1.33	23.00±1.33	19.91±1.14
	d8	22.67±1.33	23.14±1.18	20.67±1.07
	d11	22.42±1.53	23.48±1.05	20.80±1.34
	d14	20.84±1.71	23.96±1.32	21.17±1.30
Distance	d5	276.24±16.08	291.82±15.95	309.45±50.93
	d8	337.94±9.38	351.11±23.57	323.38±20.45
	d11	383.85±15.80	423.32±25.65	360.83±18.58
	d14	421.50±22.40	438.49±32.01	458.51±59.01

Data are given as mean ± SEM.