Molecular Mechanism and Potential Targets for Blocking HPV-Induced Lesion Development

Table 1

HPV oncogenes	siRNA design	Biological effects	References
HPV16 E6 and E7	Synthetic siRNA	Silencing of HPV16 E6 and E7, p53, p21 and pRb expression. Apoptosis induction in vitro.	[72]
HPV16 E6	Synthetic siRNA	Silencing of HPV16 E and E7, p53, p21 and pRb expression. Cellular proliferation inhibition in vitro. Tumor growth inhibition in vivo.	[73]
HPV18 E6	Synthetic siRNA	Silencing of HPV16 E6, p53, p21, and pRb expression. Apoptosis induction in vitro.	[74]
HPV16 E7	Synthetic siRNA in bio-adhesive gels	Silencing of HPV16 E7. Apoptosis induction in vitro.	[87]
HPV18 E6 and E7	Synthetic siRNA with chemotherapy	Silencing of HPV18 E6 and E7, as well as p53 expression. Cytotoxicity decrease in vitro.	[94]
HPV18 E6	siRNA in lentivirus with chemotherapy	Silencing of HPV18 E6 and E7, as well as p53 expression. Cytotoxicity decrease in vitro.	[86]
HPV16 E6 and E7	Synthetic siRNA in liposomes	Silencing of HPV16 E6 and E7. Apoptosis induction in vitro. Tumor growth inhibition in vivo.	[88]
HPV18 E6 and E7	siRNA with atelocollagen	Silencing of HPV18 E6 and E7, as well as p53 and pRb expression. Cellular senescence induction in vitro. Tumor growth inhibition in vivo.	[89]
HPV18 E6 and E7	Synthetic siRNA	Silencing of HPV18 E6 and E7 and cyclin A gene, as well as p53, pRb, p16, p21, and p27 expression. Apoptosis induction in vitro.	[75]
HPV18 E6 and E7	siRNA in pSUPER plasmid	Silencing of HPV16 E6 and E7, as well as p53 and pRb expression. Transcriptome expression analysis of cancer cells.	[91]
HPV16 E6 and E7	siRNA in psiCheck 2 plasmid	Silencing of HPV16 E6 and E7, as well as p53 and p21 expression. Cellular senescence induction in vitro. Tumor growth inhibition in vivo.	[95]
HPV16 E7	siRNA in pSIRE-DNR plasmid	Silencing of HPV E6 and E7, as well as p53, p21 and pRb expression. Apoptosis induction in vitro.	[96]
High risk HPV E6 and E7	siRNA in lentivirus	Silencing of HPV16 E6 and E7. p53 and p21 expression. Apoptosis induction in vitro and in vivo. High efficiency of infection of proliferation cells and quiescent cells.	[90]
HPV16 E6 and E7	siRNA in pSilencer 1.0U6-plasmid	Silencing of HPV16 E6 and E7. p53 and pRb expression. Cellular proliferation inhibition. Autophagic and apoptosis induction of tumor cells in vivo.	[97]