The Adipocyte-Derived Hormone Leptin Has Proliferative Actions on Androgen-Resistant Prostate Cancer Cells Linking Obesity to Advanced Stages of Prostate Cancer
Figure 2
Leptin activates ERK1/2 isoforms of MAPK signaling pathway in a dose-dependent manner in androgen-resistant prostate cancer cell lines (DU145 and PC-3). Three different human prostate cancer cell lines were cultured in serum-free media for 24 hrs followed by exposure to recombinant human leptin for 1 hour. Cellular extracts were fractioned onto 12% SDS-Page and Western immunoblotting performed with a rabbit polyclonal anti-phospho-p44/42 MAPK as described in Section 2. The findings are from a single experiment representative of at least 3 similar experiments.
