Journal of Oncology / 2012 / Article / Fig 1

Research Article

Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines

Figure 1

Expression of Spry1 and Spry2 proteins in a range of ovarian cancer cell lines compared with human ovarian surface epithelial cell line (HOSEpiC). (a) Western blot analysis of Spry1 and Spry2 expression in HOSEpiC, OVCAR-3, SKOV-3, 1A9, A2780, CAOV-3, OV-90, and IGROV-1 cell lines using GAPDH as a loading control. (b)-(c) Quantification of Spry expression in the cell lines yielded from at least three independent immunoblots similar to (a), using ImageQuant software. Loading amounts and quantification were normalized against GAPDH values. Moreover, the expression levels in cancer cell lines were normalized against those in HOSEpiC cells used as the normal control. The graphs represent the expression of Spry1 (b) and Spry2 (c) in arbitrary units after normalization. Altered expression of both isoforms was observed in the cancer cells as compared to the control. Data are shown as . Significant values ( ) are indicated with asterisks.

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