Initial Report on Differential Expression of Sprouty Proteins 1 and 2 in Human Epithelial Ovarian Cancer Cell Lines
Figure 2
The presence of Spry1 and 2 mRNAs evaluated by RT-PCR in OVCAR-3 (1), SKOVE-3 (2), 1A9 (3), A2780 (4), CAOV-3 (5), OV-90 (6), and IGROV-1 (7) cells compared to HOSEpiC. As seen above, Spry1 and Spry2 were differently expressed in the cancerous cells. While Spry1 mRNA was almost undetectable in CAOV-3 cells, it was expressed by other cells, being more prominent in 1A9. Spry2 mRNA was identified in all cell lines with more prominent expression in cancerous ones. The product size for Spry1 and Spry2 was 438 bases and 471 bases, respectively. -actin was used as a reference.
