Influence of Morphine on Pericyte-Endothelial Interaction: Implications for Antiangiogenic Therapy

Figure 2

Morphine coactivates PDGFR-β phosphorylation and MAPK/ERK signaling in human pericytes. Human placenta-derived pericytes were incubated with 0.1 μM morphine or 20 ng/mL PDGF-BB for indicated time or with PBS, followed by cell lysis using protease inhibitors. Lysates containing 100 μg protein were resolved on 3–15% SDS gel and Western blotted as described in the methods. (a) Representative image of five different Western blots for phospho- and total-PDGFR-β, MAPK/ERK, and STAT3 is shown. (b) Densitometric analysis of protein bands is represented as a ratio of phospho- to total-protein for each protein. Each bar is the mean ± SEM of five different blots. ^∗𝑃<0.01, ^∗∗𝑃<0.001, for each time point compared to unstimulated in each graph.