Research Article

Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of 𝜶 -Tubulin Isoforms

Figure 3

Scattergram depiction of DIGE data to generate protein expression profile of 686Tu and 686Ln cells. Each dot represents a protein detected in 686Tu and 686Ln cells. The dots are expressed as bigger pixel volume in either cell (right 𝑌 -axis), and plotted against log scale of pixel volume ratio between 686Tu and 686Ln ( 𝑋 axis), with 686Tu as standard. Two vertical lines indicate two-fold difference limits between the two cells. Left 𝑌 -axis is number of spots at certain pixel ranges. Related pink curve is actual distribution of spots versus their pixel volume ratio and green curve is normalized Gaussian distribution ( 𝑅 2 = 0 . 9 6 3 ). Arrows indicate two protein spots to be studied.
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