Research Article

Lysophosphatidic Acid Disrupts Junctional Integrity and Epithelial Cohesion in Ovarian Cancer Cells

Figure 5

Blocking MMP activity inhibits LPA-induced junction loss. (a) OVCA429 cells were treated with LPA (30 μM) for 24 hours in the presence and absence of the broad-spectrum MMP inhibitor GM6001 (25 μM) as indicated and processed for E-cadherin immunofluorescence (green). (b) E-cadherin positive cells were quantified by scoring the number of cells with two remaining borders in a minimum of 12 fields per treatment. (* 𝑃 < . 0 0 0 5 relative to control; + 𝑃 = . 0 0 7 relative to LPA).
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(a)
501492.fig.005b
(b)