Figure 6: Clonogenic survival of breast cancer cells after treatment including irradiation with or without cucurbitacin B. MCF7:5C, MDA-MB-231, and SKBR-3 cells were treated with 5 𝜇 M of cucurbitacin B for 48 hr before radiation. Following the incubation period with specific drug concentration, cells were harvested, resuspended in fresh medium, and then irradiated at 0–8 Gy. Colony formation was detected by 21 days later and survival curve was constructed. Data was a summary of three experiments.