Enhancement of cell invasiveness by NNMT via MMP-2. (a) Matrigel invasion assay was used to analyze the invasive activity. HEK293/vector and HEK293/NNMT cells were seeded into the matrigel-coated chamber and incubated at 37°C for 24 h. (b) Effects of MMP-2-neutralizing antibody or MMP-2 inhibitor OA-Hy on the invasive activity of HEK293/NNMT cells were examined. Data are presented as the mean of triplicate replications. S.D. is indicated by error bars. *; ** versus untreated HEK293/NNMT cells. (c) 786O and Caki-1 cells were transfected with 40 μmol/L of NNMT siRNA for 24 h and then incubated with or without 20 ng of MMP-2 protein in matrigel-coated chamber for another 24 h. Data presented are the mean of triplicate replications. S.D. is indicated by error bars. ** versus control cells.
