Effects of NNMT shRNA on the growth and lung metastasis of ccRCC cells in NOD-SCID mice. Western blot analysis (a) and matrigel invasion assay (b) were performed to examine NNMT and MMP-2 expression and invasive activities of 786O/NNMT shRNA and 786O/control shRNA cells. Actin was used as loading control. (c) 4 × 106 of 786O/NNMT shRNA or 786O/luciferase shRNA cells were s.c. injected into the hind limb of NOD-SCID mice . Tumor volume was measured every three days. Data are presented as the mean of triplicate replications. S.D. is indicated by error bars. ** versus control cells. (d) 1 × 106 of cells in 200 μL of serum-free DMEM medium were intravenously injected into the lateral tail vein of NOD-SCID mice . After 6 weeks of injection, the mice were sacrificed, and the metastatic nodules of lungs were counted. Data are presented as the . * versus control cells.
