Importance of SP1 in MMP-2 expression induced by NNMT. (a) Effects of SP1 siRNA on the expression of MMP-2 and SP1. Cells were transfected with 40 μmol/L of SP1 siRNA or control siRNA, and Q-PCR was performed to determine the expression levels of NNMT and MMP-2. Data are presented as the mean of triplicate replications. S.D. is indicated by error bars. *; ** versus control cells. (b) Effects of SP1 inhibitor, mithramycin, on the expression of MMP-2. Cells were treated with 12.5 or 25 nmol/L of mithramycin for 18 h, and the expression levels of MMP-2 were examined by Q-PCR. Data are presented as the mean of triplicate replications. S.D. is indicated by error bars. *; ** versus control cells.
