Role of PI3K/AKT pathway in the binding of SP1 to MMP-2 promoter. HEK293/NNMT cells were treated with or without 5 μmol/L of LY294002 or 1 μmol/L of AKT inhibitor IV for 18 h, and the association of SP1 on the SP1 binding elements of the MMP-2 promoter was determined by CHIP assay. Rabbit IgG was used as the negative control and 4% input as positive control.
