Journal of Oncology / 2012 / Article / Fig 2

Research Article

Synergistic Effect of Garcinol and Curcumin on Antiproliferative and Apoptotic Activity in Pancreatic Cancer Cells

Figure 2

Garcinol-(upper panel) or curcumin-(lower panel) treated cytosolic extracts were used to evaluate induction of apoptosis in PaCa cells. (a) BxPC-3 and (b) Panc-1 using ELISA-Histone DNA Enrichment Assay. Results demonstrate a significant dose-dependent increase in apoptotic cells in individual treatment with either agent for 48 hours. Enrichment factor was measured using subtraction of background signal. * 𝑃 < 0 . 0 5 relative to control. Apoptotic morphological changes such as abnormal nuclear morphology, reduction in cell number with apoptotic body formation, and cell shrinkage were observed in a dose responsive manner on 48 hour treatment with (c) curcumin or (d) garcinol. BxPC-3 and Panc-1 cells were fixed with DAPI stain and visualized using fluorescence microscopy with an excitation maximum at 358 nm and emission maximum at 461 nm.
709739.fig.002a
(a)
709739.fig.002b
(b)
709739.fig.002c
(c)
709739.fig.002d
(d)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.