Journal of Oncology / 2012 / Article / Fig 2

Research Article

Synergistic Effect of Garcinol and Curcumin on Antiproliferative and Apoptotic Activity in Pancreatic Cancer Cells

Figure 2

Garcinol-(upper panel) or curcumin-(lower panel) treated cytosolic extracts were used to evaluate induction of apoptosis in PaCa cells. (a) BxPC-3 and (b) Panc-1 using ELISA-Histone DNA Enrichment Assay. Results demonstrate a significant dose-dependent increase in apoptotic cells in individual treatment with either agent for 48 hours. Enrichment factor was measured using subtraction of background signal. * 𝑃 < 0 . 0 5 relative to control. Apoptotic morphological changes such as abnormal nuclear morphology, reduction in cell number with apoptotic body formation, and cell shrinkage were observed in a dose responsive manner on 48 hour treatment with (c) curcumin or (d) garcinol. BxPC-3 and Panc-1 cells were fixed with DAPI stain and visualized using fluorescence microscopy with an excitation maximum at 358 nm and emission maximum at 461 nm.

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