Dual Roles of METCAM in the Progression of Different Cancers

Figure 13

Expression of moMETCAM in mouse angiosarcoma cell lines [7]. MS1 is an immortalized mouse endothelial cell line, which expressed a barely detectable (low) level of moMETCAM and was nontumorigenic. SVR is a mouse angiosarcoma cell line, which had been transfected with H-Ras gene, also expressed moMETCAM, and was tumorigenic. K3S9, a clone derived from mouse melanoma K1735 subline number 3 which had been transfected with a moMETCAM cDNA gene, expressed a high level of moMETCAM and formed tumor efficiently in syngeneic mice (C3H). Western blot was carried out and detected by our chicken anti-moMETCAM antibody [9]. The smaller molecular weight of the moMETCAM (about 115 kDa) in angiosarcoma cell lines was probably due to less glycosylation than that in the mouse melanoma cell lines or in most human cancer cell lines (about 150 kDa).