Fluorescence In Situ Hybridization for MicroRNA Detection in Archived Oral Cancer Tissues
Figure 3
Endogenous biotin in oral cancer tissues. Following satisfactory inhibition of endogenous peroxidase using dilute hydrochloric acid solution (Figure 2), fluorescence in situ hybridization was performed using (a) 5′ biotinylated complementary DNA oligonucleotide probe against miR-146a or (b) control sample treated the same way but without the addition of probe (i.e., without any exogenous biotin). The control sample reveals endogenous biotin in oral cancer tissues. Detection used streptavidin-HRP and Cyanine 5-conjugated tyramide. Red: Cy5-tyramide showing existence of streptavidin-binding activity, blue: DAPI stained nuclei. Original magnification 200x.
