Fluorescence In Situ Hybridization for MicroRNA Detection in Archived Oral Cancer Tissues
Figure 4
miR-146a expression in oral normal squamous epithelia and squamous carcinoma. Microarrayed normal oral tissue (10 cores) and oral squamous cell carcinoma (40 cores) were evaluated using the optimized fluorescence in situ hybridization protocol as outlined in Figure 1. The probe for miR-146a is LNA modified and labeled with digoxigenin at 5′ end. (a, b) Normal oral squamous epithelia. As shown in (a) and (b), cells at the basal layer (adjacent to the white dashed line) are largely negative for miR-146a, while cells at the intermediate layer are positive. In oral squamous carcinomas, (c) well-differentiated tumors, shown here is tissue core B6, often exhibit positive miR-146a staining while (d) poorly differentiated tumors, shown is tissue core E8, tend to be negative. Red: Cy5-tyramide showing positive hybridization signals, blue: DAPI-stained nuclei. Original magnification 400x.
