Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System
Figure 5
U1A and HPV16 amplification plots from NASBA chips containing freeze-dried reagents. Freeze-dried primers and probes (U1A/HPV16) were deposited in reaction chamber 1 (250 nL) and enzymes were deposited in reaction chamber 2 (125 nL). Incubation time in reaction chamber 1 was 5 minutes. The chips were stored at room temperature for 8 and 9 days. (a) Positive control U1A/HPV 16. Channel 8 was empty. (b) CaSki (1 : 10 dilution, equivalent to 370 cell/reaction chamber on chip). Channel 6 was empty. (c) HPV 16 positive clinical specimen (Pretoria). (d) No template control. Channel 7 was empty.
