HAX1 Augments Cell Proliferation, Migration, Adhesion, and Invasion Induced by Urokinase-Type Plasminogen Activator Receptor

Figure 2

HAX1 overexpression augments HEK293/uPAR and MDA-MB-231 cell proliferation in uPAR-stimulated cells. (a) HEK293/uPAR. (b) MDA-MB-231 cells were transfected with pGEM-3Zf(+) (light bars) or pGEM-3Zf(+)∖HAX1 (dark bars). Cells were exposed to 10% FCS alone or with 100 ng/mL of EGF, uPA, or uPA-ATF. Cell proliferation was measured using MTT assay. Data is expressed as mean ± S.E of optic density (OD) readings; *** represents 𝑃<0.001 using unpaired Student’s t-test.

(a)

(b)