Antiproliferative and Apoptotic Effects of a Specific Antiprostate Stem Cell Single Chain Antibody on Human Prostate Cancer Cells
Figure 3
Flow cytometry analysis of scFv C5-I (a) and scFv C5-II (b) binding to PSCA-expressing DU-145 cells and PSCA-negative LNCaP cells (c) and (d). The both scFv antibodies bind to PSCA-expressing cell line as shown by the shift in fluorescence intensity value with the DU-145 cells compared to intensity measured from the isotype control (a) and (b). The fluorescence intensity measured from LNCaP cells was nearly identical to the values measured from isotype control or untreated cells (c) and (d). Filled area represents treated cells, white area represents untreated cells and dotted line represents isotype (M13KO7) treated cells.
