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Journal of Oncology
Volume 2014 (2014), Article ID 236482, 7 pages
http://dx.doi.org/10.1155/2014/236482
Research Article

A Case-Control Study of the Role of Human Papillomavirus in Oesophageal Squamous Cell Carcinoma in Australia

1School of Public Health and Community Medicine, The University of New South Wales, Kensington, NSW 2052, Australia
2Department of Medicine, St. Vincent's Hospital Clinical School, The University of New South Wales, Kensington, NSW 2052, Australia
3UNSW Medicine, The University of New South Wales, Kensington, NSW 2052, Australia
4Department of Microbiology and Infectious Diseases, The Royal Women’s Hospital, Parkville, VIC 3052, Australia
5Department of Obstetrics and Gynecology, Murdoch Children's Research Institute, University of Melbourne, Parkville, VIC 3010, Australia
6Department of Surgery, Princess Alexandra Hospital, The University of Queensland, St Lucia, QLD 4072, Australia
7Department of Surgery, Prince of Wales Hospital Clinical School, The University of New South Wales, Kensington, NSW 2052, Australia

Received 15 January 2014; Revised 9 April 2014; Accepted 11 April 2014; Published 28 April 2014

Academic Editor: Paul Magnus Schneider

Copyright © 2014 Surabhi S. Liyanage et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. We investigate the prevalence of human papillomavirus (HPV) in oesophageal squamous cell carcinoma (OSCC) tissues compared to oesophageal tissue from healthy controls, in an Australian cohort. Methods. We conducted a hospital-based case-control study of 99 patients with OSCC and 100 healthy controls to examine the presence of HPV DNA. Paraffin tissues were tested using the PapType high-risk HPV detection and genotyping kit and with INNO-LiPA HPV Genotyping Extra. The biopsy samples were tested for HPV using a PCR-ELISA method based on the L1 consensus primer set PGMY09-PGMY11. Results. HPV DNA of the oncogenic genotype 16 was detected in 1/99 case specimens, a rate of 1010 per 100,000 (95% CI: 30–5500). All control specimens were negative for HPV. Significantly higher rates of smoking, other aerodigestive cancers, and mortality were seen among cases than controls. A pooled analysis of this study and the only other Australian case-control study found that 9/321 cases and 0/155 controls were positive for HPV. The pooled odds ratio for HPV being a risk factor for OSCC was 9.35 (95% CI: 0.47–190.33). Conclusion. Our results suggest that in this multifactorial cancer HPV may be an additional risk factor; although a larger, better powered study is needed.