Journal of Oncology / 2015 / Article / Fig 2

Research Article

Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA

Figure 2

Esculetin treatment alters the transcript levels of AML1-ETO and C-Kit in Kausmi-1 cells. Kausmi-1 cells were used in these analyses. The results of semiquantitative PCR were for AML1-ETO and C-Kit using cDNA as template; Kausmi-1 cells were incubated with 100 μM esculetin (E) and DMSO (D) (vehicle control) along with control untreated (C) for 24 hours and 48 hours (a); results of qPCR for AML1-ETO (b) and C-Kit (c) in esculetin treated and control Kausmi-1 cells are shown. The duration of treatment is indicated on top of the lanes (I). 18SrRNA was used as an internal control for semiquantitative PCR; for qPCR 18SrRNA and GUSB were used for normalization. The data represents mean ± SD. compared to control.