Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 Cells In Vitro and In Vivo

Figure 4

Betaine induced cell cycle arrest in G₂ phase. Cell cycle study of A549 cells treated with betaine and/or C-PC. (a) First, cells were synchronized in G₁ phase by serum deprivation followed by thymidine excess (synchronized). (b) Synchronized cells were then treated for 18 h with only medium (untreated), 4% betaine, 80 μg⋅L⁻¹ of C-PC, or a combination of the two treatments (betaine + C-PC). Cell cycles analysed by flow cytometry following propidium iodide staining are shown.

(a)

(b)