Inhibition of MDM2 via Nutlin-3A: A Potential Therapeutic Approach for Pleural Mesotheliomas with MDM2-Induced Inactivation of Wild-Type P53

Figure 1

Immunohistochemical staining of the investigated MPM cell lines with antibodies directed against P53 and MDM2. NCI-H2052 shows a strong staining (Score 2) regarding P53 (a) and MDM2 (b). NCI-H2452 showed neither immunoexpression for P53 ((c), Score 0) nor for MDM2 ((d), Score 0). MSTO-211H stained positive for P53 ((e), Score 1) and MDM2 ((f), Score 1). The scale bars indicate 100 μm for pictures (a) and (b) and 500 μm for pictures (c), (d), (e) and (f).

(a) NCI-H2052, anti-P53 staining

(b) NCI-H2052, anti-MDM2 staining

(c) NCI-H2452, anti-P53 staining

(d) NCI-H2452, anti-MDM2 staining

(e) MSTO-211H, anti-P53 staining

(f) MSTO-211H, anti-MDM2 staining