Inhibition of MDM2 via Nutlin-3A: A Potential Therapeutic Approach for Pleural Mesotheliomas with MDM2-Induced Inactivation of Wild-Type P53

Figure 2

The bar chart shows the relative mRNA expression of MDM2, P53 and P14/ARF in the investigated MPM cell lines. On the x-axis the investigated cell lines are shown and the respective mRNA expression of P53, MDM2, and P14/ARF. On the y-axis the 2∧ΔCt values for the relative mRNA expression of the investigated target genes is shown after normalization against the reference gene ACTB (actin, beta). NCI-H2052 and MSTO-211H show elevated expression of MDM2, whereas NCI-H2452 showed minimal MDM2 expression. TP53 mRNA expression was reduced in NCI-H2452 compared to both other cell lines. P14/ARF expression was below the detection limit in the investigated specimens.