Overexpression of IRS-4 Correlates with Procaspase 3 Levels in Tumoural Tissue of Patients with Colorectal Cancer

Figure 1

(a) Western blot analyses of IRS-4, IRS-1, and ERK levels in RKO and HepG2 cell lines. (b) IRS-1 and IRS-4 mRNA expression levels in RKO and HepG2 cell lines were detected using qPCR; graphs depict the calculated ratios of IRS-1/IRS-4 to 18S for each sample. (c) RKO and HepG2 cells were transiently transfected with pcDNA (IRS-4) or pcDNA (negative control); cells were lysed and several proteins were analysed by western blot. A representative experiment of three performed is shown.

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