|
| Reference | Biofluid | Pathological condition | Isolation technique | EV subtype | MS-based strategy | Candidate biomarkers |
|
| Sun et al. [43] | Saliva and serum | Lung Cancer | PureEXO® isolation kit (101Bio) | Exo | ESI-QTOF MS (MaXis Impact)
LC-MS/MS (Q Exactive) | Vimentin, Phospholipid transfer protein, Annexin, Zinc-alpha-2-glycoprotein, Lactoperoxidase, Proteasome subunit alpha, Grancalcin, Cysteine-rich secretory protein 3, Calpain small subunit 1, Histone H3 |
| Sun et al. [13] | Saliva | Lung Cancer | Ultracentrifugation | Exo and MVs | LC-MS/MS (Q Exactive) | Ras GTPase-activating-like protein, BPI fold-containing family A member 1, Cornulin, Mucin-5B |
| Raimondo et al. [44] | Urine | Renal Cell Carcinoma | Iodixanol density centrifugation | Exo | LC-ESI-MS/MS (MaXis hybrid UHR-QTOF) | Matrix metalloproteinase-9, Ceruloplasmin, Podocalyxin, Dickkopf related protein 4, Carbonic Anhydrase IX, Aquaporin-1, Extracellular Matrix Metalloproteinase Inducer, Neprilysin, Dipeptidase 1, Syntenin-1 |
| Chen et al. [45] | Serum | Colorectal Cancer | Ultracentrifugation | Exo | HPLC-MS/MS (Orbitrap Fusion) | Fibronectin-1, Annexin-A1, Metalloproteinase-9, Galectin-3
binding protein, Insulin-like growth factor |
| Aqrawi et al. [11] | Saliva and tear | Sjögren’s Syndrome | Size-exclusion chromatography | Exo and MVs | LC-MS/MS (Q Exactive) | Lymphocyte-specific protein 1, Adipocyte plasma membrane-associated protein, Copine-1, Thioredoxin-dependent peroxidase reductase |
| Choi et al. [46] | Ascites | Colorectal Cancer | Iodixanol and sucrose density centrifugation | MVs | LC-ESI-MS/MS (LTQ) | Carcinoembryonic antigen cell adhesion molecule 5, Adhesion G-protein-coupled receptor E5, Galectin-3, Epithelial cell adhesion molecule, Aminopeptidase N, Trophoblast glycoprotein |
| Kittivorapart et al. [47] | Plasma | HbE/β- thalassaemia | Ultracentrifugation | Non specified | HPLC-MS/MS (Orbitrap Fusion) | Alpha hemoglobin–stabilizing protein, Catalase, Superoxide dismutase, Hemopexin, Haptoglobin |
| Shiromizu et al. [48] | Serum | Colorectal Cancer | Sucrose density centrifugation | Not specified | LC-MS/MS (Q Exactive) | Annexin-A3, A4, A5 and A11, Tenascin-N, Mucin-5B, Matrix metalloproteinase-9, Transferrin receptor protein 1 |
| Sequeiros et al. [49] | Urine | Prostate Cancer | Ultracentrifugation | Not specified | SRM-MS (5500 Q-Trap) | Adseverin, Transglutaminase-4 |
| Arbelaiz et al. [50] | Serum | Cholangiocarcinoma (CCA)
Primary Sclerosing Cholangitis (PSC)
Hepatocellular carcinoma (HCC) | Ultracentrifugation | Not specified | UPLC-MS/MS (LTQ Orbitrap XL and Synapt G2-Si) | CCA: Aminopeptidase N, Pantetheinase, Polymeric immunoglobulin receptor
PSC: Ficolin-1, Neprilysin, Fibrinogen gamma chain, Alpha-1-acid glycoprotein 1, S100A8 proteins
HCC: Galectin-3-binding protein, Polymeric immunoglobulin receptor |
| Yang et al. [51] | Seminal fluid | Prostate Cancer | Sucrose density centrifugation | Exo | HPLC-MS/MS (LTQ Orbitrap Elite) | Semenogelin-1, Phosphoglycerate dehydrogenase, Galectin-3-binding protein, Actin, Glyceraldehyde-3-phosphate dehydrogenase |
| Barnabas et al. [52] | Uterine Liquid Biopsy | Ovarian Cancer | Ultracentrifugation | MVs | LC-MS/MS (Q Exactive) | Calcium-activated chloride channel regulator 4, Involucrin, Protein S100-A14, Protein S100-A2, Serpin B5 |
| Turay et al. [53] | Plasma | Prostate Cancer | Exoquick™ Isolation Kit | Exo | HPLC-MS/MS (LCQ Deca XP) | Iroquois homeobox protein 5, Mitochondrial tumor suppressor 1 isoform 4, Trinucleotide repeat containing 6B isoform 3 |
|
