Interfering Expression of Chimeric Transcript SEPT7P2-PSPH Promotes Cell Proliferation in Patients with Nasopharyngeal Carcinoma

Figure 2

SEPT7P2-PSPH expression. (a) By RT–PCR, the SEPT7P2-PSPH fusion transcripts were verified in nine cell lines. (b) The relative expression of SEPT7P2-PSPH was higher in nasopharyngitis compared with those in NPC patients using quantitative RT-PCR (P < 0.05). (c) Full-length PSPH protein was expressed in nine cell lines by western blotting and no relevant chimeric fusion protein was detected. Expression of SEPT7P2-PSPH was knocked down by the specific siRNA. The suppression effect was verified by quantitative RT-PCR and western blotting, respectively (d, e, n = 3; two-tailed Student’s t-tests, < 0.01 and < 0.001).

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