Interaction of Anticancer Drugs with Human Organic Anion Transporter hOAT4

Figure 2

Cis effect of epirubicin hydrochloride and dabrafenib mesylate on hOAT4-mediated [³H]-ES uptake in BeWo cells. hOAT4-mediated [³H]-ES uptake was measured in stable hOAT4-expressing BeWo cells. The 4-min uptake of 300 nM [³H]-ES in the absence (control) or presence of test compounds (100 μM) was measured. Each data point represents only carrier-mediated transport after subtraction of values from parental cells. Uptake activity was expressed as percentage of uptake measured in control cells. Results shown are means ± SE (n=3). Data were analyzed statistically with ANOVA, followed by Tukey’s post hoc test. p < 0.05.