Interaction of Anticancer Drugs with Human Organic Anion Transporter hOAT4

Figure 3

Dose-dependent inhibition of hOAT4-mediated uptake by epirubicin hydrochloride and dabrafenib mesylate. Stable hOAT4-expressing COS-7 cells were incubated for 4 mins with PBS containing 300 nM [³H]-ES in the presence or absence of various concentrations of epirubicin hydrochloride (a) or dabrafenib mesylate (b). Each data point represents only carrier-mediated transport after subtraction of values from parental cells. Uptake activity was expressed as percentage of uptake measured in control cells. Results shown are means ± SE (n=3). Data were analyzed statistically with ANOVA, followed by Tukey’s post hoc test. p < 0.05. The line represents a best fit of data using nonlinear regression analysis.

(a)

(b)