Formation of Polyploid Giant Cancer Cells Involves in the Prognostic Value of Neoadjuvant Chemoradiation in Locally Advanced Rectal Cancer

Figure 4

(a) Immunocytochemical (ICC) staining of N-cadherin, vimentin, E-cadherin, Fibronectin, Snail and Slug, Twist-1, and CK7 in LoVo cells before and after irradiation (100x). (b) ICC staining of N-cadherin, vimentin, E-cadherin, fibronectin, Snail and Slug, Twist-1, and CK7 in HCT116 cells before and after irradiation (100x). (c) Western blot assay of E-cadherin, Twist-1, Snail and Slug, fibronectin, and CK7 expression in LoVo cells before and after irradiation (-C, -cytoplasm; -N, -nuclear; 100x). (d) Western blot assay of E-cadherin, Twist-1, Snail and Slug, fibronectin, and CK7 expression in HCT116 cells before and after irradiation (-C, -cytoplasm; -N, -nuclear; 100x).

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