Low Long Noncoding RNA Growth Arrest-Specific Transcript 5 Expression in the Exosomes of Lung Cancer Cells Promotes Tumor Angiogenesis

Figure 1

Low GAS5 level in serum exosomes and lung cancer tissues of mice with lung cancer. (a) Gross anatomy of the lungs from normal saline control and urethane-treated mice at different time points. (b) Representative pathological images of the lungs from control and urethane-treated mice at different time points (200×magnification, except the images from the 9th and 12th weeks which were 400×magnification). (c) Representative images of isolated exosomes by electron microscopy. (d) Exosome marker CD63 was detected by western blot. (e) lncRNAs level in mouse serum exosomes were detected by qPCR. (f) GAS5 level in serum exosomes was detected by qPCR. cel-miR-39-3p served as the external reference. (g) GAS5 level in the lungs of control and urethane-treated mice in the 12th week. (h) Correlation between serum exosomal GAS5 level and GAS5 expression in the lungs of mice was analyzed by univariate linear regression. Statistical significance, P<0.05, P<0.01.