Screening of Pleural Mesothelioma Cell Lines for Kinase Activity May Identify New Mechanisms of Therapy Resistance in Patients Receiving Platin-Based Chemotherapy

Figure 2

Top 10 influenced phosphosites during cisplatin therapy. On the y-axis, phosphosites of the respective protein are shown. Each box summarizes the results for all four cell lines, being measured in triplicates (dark blue = medium and light blue = cisplatin). To make the results comparable, the x-axis depicts a dimensionless Z-score. A significant, global reduction of tyrosine phosphosite phosphorylation could be observed. No significance for serine/threonine kinases was monitored. The figure focusses on the top 10 changes because further boxplots would be repetitive. In sum, 77 phosphosites showed significantly altered phosphorylation during cisplatin therapy. values are depicted in Suppl. Table 1.