YDJC Induces Epithelial-Mesenchymal Transition via Escaping from Interaction with CDC16 through Ubiquitination of PP2A
Figure 1
Overexpression of YDJC induces lung cancer progression in a lung cancer orthotopic mouse model. and cells were directly introduced via injection into the left lung parenchyma of NOD/SCID mice as described in the methods [16]. (a) Gross images of the lungs in NOD/SCID mice injected with and cells. (b) Hematoxylin and eosin staining of the lungs in NOD/SCID mice injected with and cells. Lungs were analyzed by hematoxylin and eosin staining. (c) Comparison of tumor areas: mice injected with versus mice injected with cells. Tumor areas were measured by intravital microscopic analysis (Axiotech Vario microscope, Zeiss, Germany). p<0.05 compared with the control group. (d) IHC staining for ERK, pERK, and PP2A. Scale bars, 20 μm. (e) IHC staining for E-cadherin and N-cadherin. Scale bars, 20 μm. In D and E, representative results are shown. Immunohistochemistry positive scores of ERK1/2, pERK1/2, and PP2A. Mean± SD; n=3. All P-values were based on two-tailed Student’s t-test.