YDJC Induces Epithelial-Mesenchymal Transition via Escaping from Interaction with CDC16 through Ubiquitination of PP2A

Figure 2

SPC induces EMT and YDJC expression is increased during SPC-induced EMT of lung cancer cells. (a) SPC time dependently induced the expression of the mesenchymal marker, N-cadherin, and YDJC and reduced the expression of E-cadherin in A549 lung cancer cells. (b) Confocal microscopic analysis of EMT markers and YDJC expression in SPC-induced EMT of A549 lung cancer cells. (c) SPC (5 μM) induced the expression of the mesenchymal marker, N-cadherin, and YDJC and reduces the expression of E-cadherin in H838 and H1299 lung cancer cells. (d) SPC induced invasion and migration of lung cancer cells. After SPC treatment, lung cancer cells were put in the upper chamber of the Transwell insert. Then, the number of cells was calculated under four randomly selected high-power fields (20×). The results are a typical example of 3 independent experiments with similar results. p<0.01 and p<0.001 compared with the control group or the SPC-treated group. (e) TGF-β1 induced YDJC expression and EMT in A549 cells. In, (a), (c), and (e), the relative expression level of each set of protein was quantified and adjusted to that of β-actin with NIH Image software.