YDJC Induces Epithelial-Mesenchymal Transition via Escaping from Interaction with CDC16 through Ubiquitination of PP2A
Figure 5
ERK2 mediates siCDC16-induced EMT. (a) MAP kinase inhibitors suppressed siCDC16-induced EMT in A549 cells. A549 cells were treated with or without MAP kinase inhibitors for 1 h in the presence of PD98059 (MEK inhibitor, 10 μM), SP600125 (JNK inhibitor, 5 μM), or SB203580 (p38 kinase inhibitor, 10 μM). (b) Confocal microscopic analysis of the inhibitory effects of MAP kinase inhibitors on siCDC16-evoked EMT in A549 cells. (c) Inhibitory effects of MAPK inhibitors on siCDC16-evoked migration and invasion. After treatment with or without MAP kinase inhibitors for 1 h followed by siCDC16, the A549 cells were added to the upper chamber of the Transwell insert. (d) Effects of siERK1 and siERK2 on siCDC16-induced EMT. (e) Confocal microscopic analysis of the inhibitory effects of siERK1 and siERK2 on siCDC16-induced EMT in A549 cells. (f) The inhibitory effects of siERK1 and siERK2 on siCDC16-evoked migration and invasion.