N-Acetyltransferase 1 Knockout Elevates Acetyl Coenzyme A Levels and Reduces Anchorage-Independent Growth in Human Breast Cancer Cell Lines

Table 1

Genomic DNA sequences of the reference (NAT14) and mutated NAT1 from each NAT1 KO clone.

Cell line	KO clone	No. of bp from start codon	Genomic sequence	No. of bp from start codon
	Reference (NAT14)		1 ATGGACATTGAAGCATATCTTGAAAGAATTGGCTATAAGA
			41 AGTCTAGGAACAAATTGGACTTGGAAACATTAACTGACAT
			81 TCTTCAACACCAGATCCGAGCTGTTCCCTTTGAGAACCTT
			121 AACATCCATTGTGGGGATGCCATGGACTTAGGCTTAGAGG
			161 CCATTTTTGATCAAGTTGTGAGAAGAAATCGGGGTGGATG
			201 GTGTCTCCAGGTCAATCATCTTCTGTACTGGGCTCTGACC
			241 ACTATTGGTTTTGAGACCACGATGTTGGGAGGGTATGTTT
			281 ACAGCACTCCAGCCAAAAAATACAGCACTGGCATGATTCA
			321 CCTTCTCCTGCAGGTGACCATTGATGGCAGGAACTACATT
			361 GTCGATGCTGGGTTTGGACGCTCATACCAGATGTGGCAGC
			401 CTCTGGAGTTAATTTCTGGGAAGGATCAGCCTCAGGTGCC
			441 TTGTGTCTTCCGTTTGACGGAAGAGAATGGATTCTGGTAT
			481 CTAGACCAAATCAGAAGGGAACAGTACATTCCAAATGAAG
			521 AATTTCTTCATTCTGATCTCCTAGAAGACAGCAAATACCG
			561 AAAAATCTACTCCTTTACTCTTAAGCCTCGAACAATTGAA
			601 GATTTTGAGTCTATGAATACATACCTGCAGACATCTCCAT
			641 CATCTGTGTTTACTAGTAAATCATTTTGTTCCTTGCAGAC
			681 CCCAGATGGGGTTCACTGTTTGGTGGGCTTCACCCTCACC
			721 CATAGGAGATTCAATTATAAGGACAATACAGATCTAATAG
			761 AGTTCAAGACTCTGAGTGAGGAAGAAATAGAAAAAGTGCT
			801 GAAAAATATATTTAATATTTCCTTGCAGAGAAAGCTTGTG
			841 CCCAAACATGGTGATAGATTTTTTACTATTTAG
MDA-MB-231	gRNA #2	76	GACATTCTTCAACACCAGATC-GAGCTGTT	105
MDA-MB-231	gRNA #5	21	TGAAAGAATTGGCTATAAGAAG--TAGGAA	50
MCF-7	gRNA #2	91	CAGA-----------------------------TGTGGG	135
MCF-7	gRNA #5	31	GGCTATAAGAA-TCTAGGAACAAATTGGAC	60
MCF-7	gRNA #5	31	GGCTATAAGAAGA-----AACAAATTGGAC	60
ZR-75-1	gRNA #2	76	GACATTCTTCAACACCAGATACCGAGCTGTT (mutated nucleotide in bold face font)	105