Research Article
N-Acetyltransferase 1 Knockout Elevates Acetyl Coenzyme A Levels and Reduces Anchorage-Independent Growth in Human Breast Cancer Cell Lines
Table 2
Amino acid sequences of reference (NAT1 4) and mutated NAT1 from each NAT1 KO clone.
| | Cell line | KO clone | Amino acid sequence | No. of total amino acids |
| | | NAT1 4 (Reference) | MDIEAYLERIGYKKSRNKLDLETLTDILQHQIRAVPFENLNIHCGDAMDLGLEAIFDQVVRRNRGGWCLQVNHLLYWALTTIGFETTMLGGYVYSTPAKKYSTGMIHLLLQVTIDGRNYIVDAGFGRSYQMWQPLELISGKDQPQVPCVFRLTEENGFWYLDQIRREQYIPNEEFLHSDLLEDSKYRKIYSFTLKPRTIEDFESMNTYLQTSPSSVFTSKSFCSLQTPDGVHCLVGFTLTHRRFNYKDNTDLIEFKTLSEEEIEKVLKNIFNISLQRKLVPKHGDRFFTI stop | 290 | | MB-MDA-231 | gRNA #2 clone | MDIEAYLERIGYKKSRNKLDLETLTDILQHQIELFPLRTLTSIVGMPWT stop | 49 | | MB-MDA-231 | gRNA #5 clone | MDIEAYLERIGYKK stop | 14 | | MCF-7 | gRNA #2 clone | MDIEAYLERIGYKKSRNKLDLETLTDILQHQIVGMPWT stop | 38 | | MCF-7 | gRNA #5 clone (allele 1) | MDIEAYLERIGYKNLGTNWTWKH stop | 23 | | MCF-7 | gRNA #5 clone (allele 2) | MDIEAYLERIGYKKKQIGLGNIN stop | 23 | | ZR-75-1 | gRNA #2 clone | MDIEAYLERIGYKKSRNKLDLETLTDILQHQIPSCSL stop | 37 |
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