PD-L1 Expression in Human Breast Cancer Stem Cells Is Epigenetically Regulated through Posttranslational Histone Modifications

Figure 1

EMT markers and PD-L1 expression in MCF-7 and BT-549 breast cancer cells and tumorspheres. MCF-7 and BT-549 cells were cultured in Cancer Stem Premium™ media for 5-10 days. Representative image shows the tumorspheres formed from MCF-7 and BT-549 cell lines (a). Western blots show the expression of stemness markers in MCF-7 and BT-549 cell lines and tumorspheres (b). Representative flow cytometric plots show the expression of PD-L1 in MCF-7 and BT-549 cell lines and tumorspheres (c). Bar plots show the PD-L1 mean fluorescence intensity in MCF-7 and BT-549 cell lines and tumorspheres (d). Bar plots showing the relative expression of PD-L1 in MCF-7 and BT-549 cell lines and tumorspheres (e). All data were normalized to β-actin.

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