PD-L1 Expression in Human Breast Cancer Stem Cells Is Epigenetically Regulated through Posttranslational Histone Modifications

Figure 3

Analysis of H3K9me3, H3K27me3, and H3K4me3 distribution in the PD-L1 promoter of MCF-7 and BT-549 cell lines and tumorspheres. Chromatin prepared from MCF-7 and BT-549 cell lines and tumorspheres were precipitated using H3K9me3, H3K27me3, and H3K4me3 antibodies and IgG as negative control. qPCR was performed on the precipitated DNA using the PD-L1 primer and data were normalized to input. Bar plots show the H3K9me3, H3K27me3, and H3K4me3 distribution in MCF-7 (a) and BT-549 (b) PD-L1 promoters. Representative Western blots show the expression of HAT and HDAC in MCF-7 and BT-549 cell lines and tumorspheres (c). Bar plots show the HDAC1 and HAT in MCF-7 (d) and BT-549 (e) cell lines and tumorspheres. Data are representative of two independent experiments.

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