ANXA2 Silencing Inhibits Proliferation, Invasion, and Migration in Gastric Cancer Cells
Figure 5
ANXA2 siRNA suppressed the ANXA2 mRNA and protein expression levels in gastric cancer cell lines. (a) Quantitative real-time PCR analyzed ANXA2 mRNA levels in different gastric cancer cell lines including SGC-7901, GES-1, BGC-823, MKN-45, and AGS. ∗P<0.05, ∗∗P<0.01 compared to GES-1. (b) The expression level of ANXA2 protein in SGC-7901, GES-1, BGC-823, MKN-45, and AGS cells was determined using western blot. GAPDH was utilized as the internal control. (c) Bright and GFP fluorescent field of AGS cell 72 h after infection with negative control (middle panel) or lentivirus containing ANXA2-RNAi (knock-down, right panel), magnification, 200×, scale bar, 100 μm. (d) Quantitative real-time PCR assessment of ANXA2 mRNA levels in AGS siRNA-infected cells compared to control and negative control cells, ∗∗P<0.01. (e) Western blot analysis of ANXA2 protein expression in ANXA2 knock-down cells compared to control and negative control cells.