miR-199a-5p Represses Protective Autophagy and Overcomes Chemoresistance by Directly Targeting DRAM1 in Acute Myeloid Leukemia

Figure 2

(a) qRT-PCR analysis of transfection efficiency of miR-199a-5p mimic in K562/ADM cells and miR-199a-5p inhibitor in K562 cells. (n = 3; ). (b) K562/ADM cells were transfected with miR-199a-5p mimic or negative control (NC) mimic and subsequently treated with 140 μg/ml of ADM for 48 h, and cells viability was evaluated by the CCK-8 assay (n = 3; ). (c) K562 cells were transfected with miR-199a-5p inhibitor or NC inhibitor with 1.5 μg/ml of ADM for 48 h, and cells viability was evaluated by the CCK-8 assay (n = 3; ).

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