miR-199a-5p Represses Protective Autophagy and Overcomes Chemoresistance by Directly Targeting DRAM1 in Acute Myeloid Leukemia

Figure 8

(a) RT-PCR and western blot analysis were conducted to examine basic DRAM1 expression in K562 and K562/ADM cells (n = 3; ). (b) RT-PCR and western blot analysis were performed to determine DRAM1 expression when K562/ADM cells were transfected with miR-199a-5p mimic or mimic NC (n = 3; ). (c) RT-PCR and western blot analysis were performed to determine DRAM1 expression when K562 cells were transfected with miR-199a-5p inhibitor or inhibitor NC (n = 3; ). (d) Dual luciferase reporter assay of K562/ADM cells cotransfected firefly luciferase construct containing DRAM1 3′-UTR or mutant DRAM1 3′-UTR along with miR-199a-5p mimic or mimic NC. Normalized luciferase activity of empty control vector- (pmirGLO-) transfected cells is set to 1.0 (n = 3; ).

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