Journal of Oncology / 2019 / Article / Fig 9

Research Article

miR-199a-5p Represses Protective Autophagy and Overcomes Chemoresistance by Directly Targeting DRAM1 in Acute Myeloid Leukemia

Figure 9

(a) K562/ADM cells were transiently transfected with three DRAM1 siRNAs or the siNC. Downregulation of DRAM1 was confirmed by both qPCR and western blot (n = 3; ). (b) Western blot was used to examine LC3II/I expression after K562/ADM cells were transfected with DRAM1-siRNA (n = 3; ). (c) CCK-8 assay was used to examine the cell viability after K562/ADM cells were transfected with DRAM1-siRNA and subsequently treated with 140 μg/ml of ADM for 48 h (n = 3; ).
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