Expression Analysis of the Mediators of Epithelial to Mesenchymal Transition and Early Risk Assessment of Therapeutic Failure in Laryngeal Carcinoma

Figure 2

Epithelial-mesenchymal transition is prominent in invasive LSCC tissues under NOS2/NF-κB inflammatory conditions. (a) Representative immunohistochemical sections for NOS2 expression in normal epithelium, in situ carcinoma, and invasive carcinoma (magnification ×40) (yellow arrow: normal epithelium and black arrow: tumor cells). (b) Immunohistochemical scoring comparison for NOS2 expression in normal epithelium (n = 10), in situ carcinoma (n = 9), and invasive carcinoma (n = 20). Data are shown as mean ± SD. One-way ANOVA test followed by Bonferroni's multiple comparison test. (c) Pearson correlation analysis between NOS2 and EMT markers expression in invasive LSCC (n = 20). (d) Plasmatic NO₂⁻ levels in healthy donors (HD) (n = 9) and invasive LSCC patients (n = 20) were assessed using the Griess method, and t-test was performed for statistical analysis; data are shown as mean ± SD. (e) Representative immunohistochemical sections for NF-κB (p65) expression in normal laryngeal tissues and in invasive carcinomas (magnification ×40) (yellow arrow: normal epithelium and black arrow: tumor cells). (f) Pearson correlation analysis between NF-κB (p65) nuclear status and EMT markers expression in invasive LSCC (n = 20). (g) MCA of the relation between EMT, NOS-2, and NF-κB (p65).

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