Roles of the Phosphorylation of Transcriptional Factors in Epithelial-Mesenchymal Transition
Figure 1
Structures of the core EMT-TFs. (a) Snail has N-terminal SNAG domain and C-terminal DNA binding domain of four C2H2 zinc finger (ZF) motifs. Nuclear export sequences (NES) and a serine-rich domain (SRD) are located at the central region of Snail. Slug has N-terminal SNAG domain as a transcriptional repressor, proline-rich SLUG domain in the central region, and C-terminal five zinc finger motifs. (b) ZEB has two zinc finger clusters that are N-terminal zinc finger (NZF) and C-terminal zinc finger (CZF) and a centrally located homeodomain (HD). The corepressors bind to the protein binding domain of ZEB1, which are CtBP interaction domain (CID) at C-terminus, Smad interaction domain (SID) and homeodomain (HD) at the center, and CAF/p300 binding domain (CBD) at N-terminus of ZEB1. (c) Twist1 has a DNA-binding basic region (amino acids 109–121) and a bHLH domain (amino acids 122-163) and Twist WR domain (amino acids 182-202) for the transcriptional activity. SRD, serine-rich domain; ZF, zinc finger domain; NES, nuclear export sequence; CBD, coactivator binding domain; SID, Smad interaction domain; BD, homeodomain; OD, CtBP interaction domain; NLS, nuclear localization signals.