Gastrointestinal Carcinoma with Plasmacytoid Morphology: Positivity for c-MET, Arylsulfatase, and Markers of Epithelial-Mesenchymal Transition, as Indicators of Aggressivity

Table 2

The immunohistochemical markers used in the study.

Marker	Supplier	Clone	Dilution	Scoring
CD138	Dako, Glostrup, Denmark	MI 15	RTU	≥50% cytoplasmic or membrane staining
CDX2	Dako	DAK-CDX2	RTU	≥1% nuclear staining
CEA	Thermo Scientific, San Diego, CA, USA	Ab3	1:200	≥1% cytoplasmic staining
CK AE1/AE3	Dako	AE1/AE3	1:100	≥1% cytoplasmic or membrane staining
CK20	Thermo Scientific	Q6	1:100	≥1% cytoplasmic or membrane staining
CK7	Thermo Scientific	OV-TL 12/30	1:100	≥1% cytoplasmic or membrane staining
MLH-1	Leica	ESO5	1:50	≥1% nuclear staining
MSH-2	Leica	25D12	1:50	≥1% nuclear staining
PMS-2	Leica	Monoclonal	1:50	≥1% nuclear staining
MSH-6	Leica	Monoclonal	1:50	≥1% nuclear staining
SLUG	Santa Cruz	Polyclonal	1:100	Cytoplasmic positivity – 1% cut-off
E-cadherin	Dako	NCH-38	1:50	Membrane positivity – 5% cut-off
β-catenin	Leica	17 C2	1:50	Membrane, cytoplasmic or nuclear positivity – 5% cut-off
N-cadherin	Dako	6G11	1:100	Membrane or cytoplasmic positivity – 1% cut-off
Vimentin	Dako	V9	1:800	Cytoplasmic positivity – 10% cut-off
c-met	Abcam, Cambridge, UK	Monoclonal	1:2000	Cytoplasmic positivity – 5% cut-off
VSIG	Sigma Aldrich	Polyclonal	1:2500	Cytoplasmic or membrane positivity – 5% cut-off
S100	Thermo Scientific	Polyclonal	1:8000	Cytoplasmic positivity – 5% cut-off
SMA	Dako	1A4	RTU	Cytoplasmic positivity – 1% cut-off
CD44	Leica	DF1485	1:50	Cytoplasmic or membrane positivity – 10% cut-off
Maspin	Santa Cruz	H-130	1:25	Cytoplasmic or nuclear positivity – 5% cut-off
VEGF-A	Novocastra	VG1	1:50	Cytoplasmic positivity – 5% cut-off
Synaptophysin	Dako	DAK-SYNAP	RTU	Cytoplasmic or membrane positivity – 5% cut-off
Chromogranin	Dako	DAK A3	RTU	Cytoplasmic or membrane positivity – 5% cut-off
NSE	Dako	M0873	1:100	Cytoplasmic or membrane positivity – 5% cut-off
ER	Dako	1D5	RTU	Nuclear positivity - 1% cut-off
PR	Dako	PgR636	RTU	Nuclear positivity - 1% cut-off
HER-2	Dako	Polyclonal	1:200	Membrane positivity – HercepTestTM guidelines
Melan A	Dako	A103	RTU	Cytoplasmic positivity – 1% cut-off
HMB45	Cell Marque	Monoclonal	RTU	Cytoplasmic positivity – 1% cut-off