Research Article
Circulating Cancer Stem Cell-Derived Extracellular Vesicles as a Novel Biomarker for Clinical Outcome Evaluation
Table 1
Reagent list-Panel 1-Panel 2-Panel 3.
| | Reagent | Fluorochrome/reagent | Vendor | Clone | Catalog number | Amount per test |
| | Panel 1 | | CD133/2 | PE | Miltenyi Biotec | 293C3 | 130-113-186 | 1 μl | | EpCAM | PerCP-Cy5.5 | BD Biosciences | (EBA-1) | 347199 | 5 μl | | CD45 | BV510 | BD Biosciences | HI30 | 626266 (custom kit) | 5 μl |
| | Panel 2 | | CD41a | PE | BD Biosciences | HIP8 | 626266 (custom kit) | 5 μl | | CD31 | PE-Cy7 | BD Biosciences | WM59 | 626266 (custom kit) | 5 μl | | CD45 | BV510 | BD Biosciences | HI30 | 626266 (custom kit) | 5 μl |
| | Panel 3 | | CD90 | FITC | BD Biosciences | 5E10 | 555595 | 1 μl | | CD29 | PE | BD Biosciences | MAR4 | 555443 | 3 μl | | CD45 | BV510 | BD Biosciences | HI30 | 626266 (custom kit) | 5 μl | | CD235a | BV421 | BD Biosciences | GA-R2 (HIR2) | 562938 | 5 μl |
|
|
FITC = fluorescein isothiocyanate; PE = R-phycoerythrin; PerPC-Cy5.5 = peridinin-chlorophyll proteins-cyanine 5.5; PE-Cy7 = PE-Cyanine 7, BV = Brilliant Violet.
|
